Crude enzyme extract from Lactobacillus bulgaricus was purified by ammonium sulfate salting-out, ultrafiltration and gel fractionation chromatography. Purification of about 23.00-fold was achieved. 保加利亚乳杆菌亚油酸异构酶的粗酶液经硫酸铵盐析、超滤、凝胶过滤层析等步骤进行分离纯化后,酶的纯化倍数为23.00。
Immunoglobulin G ( IgG) in chicken serum was separated and purified by salting-out of ammonium sulfate and column chromatography. 经硫酸铵盐析沉淀、柱色谱分离纯化得到鸡血清IgG。